Novel sericin-based hepatocyte serum-free medium and sericin’s effect on hepatocyte transcriptome

Hepatocyte Selection Medium

Fibrinogen synthesis in serum-free hepatocyte cultures: stimulation by glucocorticoids.

Fibrinogen synthesis was investigated in cultures of chicken embryo hepatocytes initiated and maintained in chemically defined, serum-free medium. 11-Hydroxy glucocorticoids caused a 3-fold stimulation of fibrinogen synthesis. Half-maximal stimulation was achieved with 1 nM corticosterone or hydrocortisone, as compared with only 0.1 nM dexamethasone. Increased fibrinogen production in the prese...

Presence of urokinase in serum-free primary rat hepatocyte cultures and its role in activating hepatocyte growth factor.

Serum-free rat hepatocyte cultures can be stimulated to divide by the inactive, single-chain form of hepatocyte growth factor (scHGF), suggesting that hepatocytes contain a protein that can cleave scHGF to its biologically active, two-chain (tcHGF) form. We added radiolabeled scHGF to serum-free cultures and confirmed that tcHGF was being generated. Because scHGF can be cleaved to tcHGF by plas...

Presence of Urokinase in Serum-free Primary Rat Hepatocyte Cultures and Its Role in Activating Hepatocyte Growth Factor1

Serum-free rat hepatocyte cultures can be stimulated to divide by the inactive, single-chain form of hepatocyte growth factor (scHGF), suggest ing that hepatocytes contain a protein that can cleave scHGF to its biologically active, two-chain (tcHGF) form. We added radiolabeled scHGF to serum-free cultures and confirmed that tcHGF was being generated. Because scHGF can be cleaved to tcHGF by pla...

Effect of glucocorticoids, insulin and a growth promoting tripeptide on the biosynthesis of plasma proteins in serum-free hepatocyte cultures.

The effect of cortisol, dexamethasone, insulin and a liver cell growth promoting tripeptide on the secretion of plasma proteins into the medium of rat hepatocytes in monolayer cultures was studied. Cortisol and dexamethasone resulted in equal to or approximately 2.5-fold increase in the fibrinogen synthesis with general suppression of albumin and alpha-lipoprotein synthesis. On the other hand, ...